NOTE	1.	Primers are ordered on the chromosomal map starting from the tip of short arm (p) to the tip of long (q) arm within a chromosome. Clicking on the marker name will bring you more information about the marker.
	2.	For each primer pair in Set III, Set IV and Set V, only the forward primer is labeled with fluorescent dye. Fluorescent labeled primer information for Sets I/II are indicated along with detailed primer information (click on marker name).
	3.	The suggested multiplex groups are based on information kindly provided by Rohrer et al. "Mapping 28 erythrocyte antigen, plasma protein and enzyme polymorphisms using an efficient genomic scan of the porcine genome" (Animal Genetics, 28:323-330).
	4.	All primers were made and therefore available in groups. Clicking on the group name will bring you the primer set information.
	5.	Operon recommendation for storage: It is recommended that the oligos be brought up in a slightly basic solution (i.e. pH=8). If they are brought up at pH<7 (deionized water is pH=5), it has been shown that the oligo can begin to degrade as soon as within a couple of weeks.

Chromosomal Locations	Marker Name1	Fluorescent Dye2	PCR Annealing Temperature	Multiplex Group3	Primer Set4
Chr. 1	SW1824	Tet	60	28	Set IV
Chr. 1	SW552	Hex	60	-	Set VI
Chr. 1	SW1515	Hex	60	34	Set V
Chr. 1	SW64	Tet	58	-	Set I/II
Chr. 1	S0316	Fam	62	-	Set V
Chr. 1	SW1851	Tet	62	-	Set III
Chr. 1	SW2130	Tet	60	-	Set VI
Chr. 1	CGA	Hex	55	-	Set I/II
Chr. 1	SW781	Hex	55	-	Set I/II
Chr. 1	SW2035	Fam	60	-	Set VI
Chr. 1	SW2185	Fam	55	-	Set IV
Chr. 1	S0313	Hex	55	-	Set I/II
Chr. 1	S0113	Tet	60	-	Set I/II
Chr. 1	SWR982	Fam	58	-	Set VI
Chr. 1	S0155	Fam	55	-	Set I/II
Chr. 1	SW974	Fam	58	9	Set IV
Chr. 1	S0320	Tet	58	-	Set V
Chr. 1	S0112	Tet	55	46	Set I/II
Chr. 1	S0056	Hex	58	-	Set V
Chr. 1	SW1301	Fam	58	26	Set IV
Chr. 2	SW2443	Hex	62	-	Set III
Chr. 2	SW2623	Tet	60	41	Set IV
Chr. 2	SW256	Fam	62	-	Set I/II
Chr. 2	SWR1910	Hex	65	-	Set VI
Chr. 2	SWR783	Tet	62	-	Set III
Chr. 2	S0141	Hex	55	-	Set I/II
Chr. 2	SW240	Tet	55	40	Set I/II
Chr. 2	SW1201	Tet	58	-	Set IV
Chr. 2	SW1564	Fam	58	-	Set V
Chr. 2	SW1026	Tet	60	44	Set IV
Chr. 2	SW395	Fam	55	-	Set I/II
Chr. 2	SW1883	Tet	58	-	Set VI
Chr. 2	S0010	Hex	55	-	Set I/II
Chr. 2	S0226	Fam	55	-	Set I/II
Chr. 2	SWR2157	Hex	60	31	Set IV
Chr. 2	SW2514	Hex	62	-	Set III
Chr. 2	S0378	Hex	55	-	Set I/II
Chr. 2	SW2192	Hex	65	10	Set IV
Chr. 2	S0036	Hex	58	13	Set III
Chr. 3	SW274	Tet	60	-	Set IV
Chr. 3	SW2021	Tet	65	-	Set VI
Chr. 3	SW72	Fam	58	-	Set I/II
Chr. 3	SWR1637	Hex	65	-	Set IV
Chr. 3	S0206	Fam	60	-	Set I/II
Chr. 3	SW2618	Hex	58	49	Set IV
Chr. 3	SW902	Fam	55	41	Set I/II
Chr. 3	S0164	Tet	55	-	Set I/II
Chr. 3	SW2570	Tet	58	-	Set V
Chr. 3	S0216	Tet	55	-	Set I/II
Chr. 3	SW2408	Fam	58	11	Set IV
Chr. 3	S0002	Hex	62	-	Set I/II
Chr. 3	SW1327	Fam	58	-	Set VI
Chr. 3	SW349	Hex	62	-	Set IV
Chr. 3	S0165	Fam	62	-	Set III
Chr. 4	S0011	Hex	55	-	Set V
Chr. 4	SW2404	Hex	62	33	Set III
Chr. 4	S0227	Hex	55	-	Set I/II
Chr. 4	SW489	Hex	55	-	Set VI
Chr. 4	S0301	Fam	55	11	Set I/II
Chr. 4	S0001	Fam	55	-	Set I/II
Chr. 4	SW35	Fam	58	-	Set III
Chr. 4	SW839	Fam	62	-	Set III
Chr. 4	S0217	Tet	62	-	Set III
Chr. 4	S0073	Fam	55	-	Set III
Chr. 4	S0214	Tet	55	32	Set I/II
Chr. 4	SW445	Tet	58	-	Set III
Chr. 4	S0097	Tet	58	-	Set I/II
Chr. 4	SW856	Tet	62	48	Set IV
Chr. 5	SW491	Tet	60	-	Set VI
Chr. 5	SW1482	Tet	55	-	Set III
Chr. 5	SWR453	Hex	55	-	Set I/II
Chr. 5	SW2425	Tet	58	36	Set V
Chr. 5	SW2	Fam	55	-	Set III
Chr. 5	SW1134	Tet	60	-	Set III
Chr. 5	S0005	Tet	58	-	Set I/II
Chr. 5	SW1468	Tet	55	2	Set IV
Chr. 5	SW152	Fam	62	-	Set VI
Chr. 5	SW1200	Fam	60	43	Set IV
Chr. 5	IGF1	Fam	58	-	Set I/II
Chr. 5	SW995	Fam	58	21	Set III
Chr. 5	SW378	Tet	60	45	Set III
Chr. 5	SW967	Hex	58	43	Set I/II
Chr. 6	S0099	Fam	68	-	Set V
Chr. 6	S0035	Fam	65	-	Set III
Chr. 6	SW2406	Tet	58	42	Set III
Chr. 6	SW1353	Hex	58	-	Set V
Chr. 6	SW1841	Fam	58	-	Set VI
Chr. 6	SW1057	Hex	58	-	Set I/II
Chr. 6	S0087	Tet	58	32	Set I/II
Chr. 6	SW1067	Hex	60	-	Set VI
Chr. 6	S0220	Fam	60	-	Set I/II
Chr. 6	SW122	Fam	55	53	Set I/II
Chr. 6	SW316	Hex	58	-	Set III
Chr. 6	S0003	Hex	55	-	Set I/II
Chr. 6	S0228	Tet	55	-	Set I/II
Chr. 6	SW1881	Fam	58	29	Set IV
Chr. 6	SW322	Tet	62	52	Set III
Chr. 6	SW2419	Tet	58	54	Set III
Chr. 7	SW2564	Tet	58	-	Set VI
Chr. 7	S0025	Fam	55	16	Set I/II
Chr. 7	SWR1343	Fam	60	-	Set IV
Chr. 7	SW1354	Tet	58	24	Set IV
Chr. 7	S0064	Fam	55	-	Set I/II
Chr. 7	SW1369	Hex	60	23	Set IV
Chr. 7	SW472	Tet	58	-	Set III
Chr. 7	S0102	Fam	55	-	Set I/II
Chr. 7	SW175	Hex	55	-	Set I/II
Chr. 7	S0066	Tet	55	-	Set I/II
Chr. 7	SW352	Tet	55	-	Set III
Chr. 7	SW632	Tet	58	-	Set I/II
Chr. 7	S0101	Hex	60	25	Set I/II
Chr. 7	S0212	Fam	55	-	Set III
Chr. 7	SW764	Tet	60	47	Set III
Chr. 8	SW2410	Hex	55	-	Set III
Chr. 8	S0098	Tet	65	-	Set V
Chr. 8	SW905	Fam	55	-	Set I/II
Chr. 8	SW1702	Tet	60	-	Set IV
Chr. 8	SWR1101	Fam	62	-	Set VI
Chr. 8	SW1029	Hex	60	30	Set IV
Chr. 8	S0017	Hex	55	-	Set I/II
Chr. 8	S0086	Fam	55	35	Set I/II
Chr. 8	S0069	Hex	62	-	Set IV
Chr. 8	S0225	Hex	55	-	Set I/II
Chr. 8	SW763	Hex	62	8	Set IV
Chr. 8	SW1312	Hex	58	-	Set VI
Chr. 8	SW61	Hex	55	65	Set I/II
Chr. 8	OPN (SPP1)	Hex	60	-	Set I/II
Chr. 8	S0178	Tet	58	-	Set I/II
Chr. 9	SW983	Hex	60	41	Set III
Chr. 9	SW21	Hex	60	6	Set III
Chr. 9	S0024	Hex	55	-	Set V
Chr. 9	SW911	Fam	55	54	Set I/II
Chr. 9	SWR1848	Tet	60	-	Set VI
Chr. 9	SW2401	Fam	58	46	Set IV
Chr. 9	SW940	Hex	58	-	Set III
Chr. 9	SW54	Fam	63	-	Set V
Chr. 9	SW539	Fam	55	-	Set I/II
Chr. 9	S0019	Hex	55	-	Set III
Chr. 9	S0295	Fam	58	-	Set III
Chr. 9	SW2093	Tet	62	55	Set VI
Chr. 9	SW174	Tet	55	-	Set I/II
Chr. 9	SW749	Tet	58	-	Set III
Chr. 10	SW830	Fam	55	5	Set I/II
Chr. 10	S0038	Hex	62	-	Set III
Chr. 10	SW249	Hex	55	-	Set I/II
Chr. 10	SW1894	Hex	58	-	Set V
Chr. 10	SW497	Fam	58	4	Set IV
Chr. 10	SWC19	Hex	58	28	Set IV
Chr. 10	SW173	Tet	55	-	Set I/II
Chr. 10	S0070	Hex	55	-	Set I/II
Chr. 10	SW1041	Hex	58	-	Set III
Chr. 10	SW920	Fam	55	46	Set I/II
Chr. 10	SW951	Hex	58	-	Set I/II
Chr. 10	SW1626	Tet	62	-	Set V
Chr. 10	SW2067	Hex	55	-	Set III
Chr. 11	S0385	Hex	55	-	Set III
Chr. 11	SW1460	Fam	58	42	Set VI
Chr. 11	SW2008	Hex	60	-	Set III
Chr. 11	S0182	Fam	50	-	Set V
Chr. 11	S0071	Hex	55	23	Set I/II
Chr. 11	SW435	Tet	55	-	Set I/II
Chr. 11	S0230	Fam	55	-	Set I/II
Chr. 11	S0386	Tet	48	-	Set I/II
Chr. 11	S0009	Fam	55	40	Set I/II
Chr. 11	SW1377	Tet	60	-	Set V
Chr. 11	SW703	Hex	58	36	Set IV
Chr. 11	SW2413	Fam	62	-	Set IV
Chr. 12	SW2490	Hex	58	25	Set IV
Chr. 12	S0143	Tet	55	-	Set I/II
Chr. 12	S0229	Fam	58	-	Set IV
Chr. 12	SW957	Hex	58	18	Set III
Chr. 12	S0083	Hex	58	-	Set V
Chr. 12	SW874	Hex	55	37	Set I/II
Chr. 12	SW168	Hex	62	-	Set VI
Chr. 12	S0090	Fam	55	4	Set I/II
Chr. 12	SW1962	Fam	60	-	Set VI
Chr. 12	S0106	Hex	55	-	Set I/II
Chr. 12	SW605	Fam	55	-	Set I/II
Chr. 12	SWR1021	Hex	60	-	Set IV
Chr. 13	S0219	Hex	55	-	Set I/II
Chr. 13	SWR1941	Tet	62	-	Set IV
Chr. 13	SW935	Tet	58	12	Set IV
Chr. 13	SW344	Tet	55	-	Set V
Chr. 13	SW864	Fam	60	30	Set IV
Chr. 13	SWr1008	Hex	62	-	Set I/II
Chr. 13	S0068	Tet	55	-	Set I/II
Chr. 13	SW225	Tet	55	-	Set III
Chr. 13	SW398	Hex	55	-	Set I/II
Chr. 13	SW1056	Tet	62	50	Set I/II
Chr. 13	SW2440	Tet	55	-	Set VI
Chr. 13	S0289	Fam	62	-	Set V
Chr. 13	SW769	Fam	55	-	Set I/II
Chr. 13	S0215	Hex	55	22	Set I/II
Chr. 14	SW857	Hex	55	-	Set I/II
Chr. 14	SW1125	Tet	60	30	Set IV
Chr. 14	SW295	Tet	55	-	Set I/II
Chr. 14	SW2612	Tet	60	34	Set IV
Chr. 14	SW210	Fam	55	-	Set I/II
Chr. 14	SW63	Hex	62	-	Set VI
Chr. 14	S0007	Hex	55	-	Set I/II
Chr. 14	SW77	Hex	62	-	Set V
Chr. 14	SW761	Tet	60	-	Set III
Chr. 14	SW1557	Tet	58	-	Set III
Chr. 14	SW2515	Fam	55	-	Set I/II
Chr. 14	SWC27	Fam	58	15	Set IV
Chr. 15	S0355	Fam	55	-	Set I/II
Chr. 15	SW1204	Hex	58	-	Set III
Chr. 15	S0004	Hex	55	-	Set III
Chr. 15	SW1111	Tet	55	-	Set I/II
Chr. 15	SW964	Tet	58	7	Set IV
Chr. 15	S0088	Tet	58	-	Set IV
Chr. 15	SW1683	Tet	58	9	Set IV
Chr. 15	SW936	Fam	58	-	Set I/II
Chr. 15	SW906	Tet	55	-	Set I/II
Chr. 15	SW1983	Hex	55	3	Set IV
Chr. 15	SW1262	Fam	60	-	Set VI
Chr. 15	SW1119	Hex	60	52	Set I/II
Chr. 16	S0111	Hex	58	12	Set IV
Chr. 16	SW742	Hex	60	-	Set III
Chr. 16	S0006	Tet	58	-	Set III
Chr. 16	S0298	Tet	60	-	Set III
Chr. 16	S0026	Hex	55	-	Set I/II
Chr. 16	SW2517	Fam	58	40	Set IV
Chr. 16	SW1897	Fam	62	13	Set V
Chr. 16	S0061	Hex	55	-	Set I/II
Chr. 17	SW335	Tet	55	29	Set IV
Chr. 17	SWr1004	Fam	60	-	Set I/II
Chr. 17	SW24	Tet	58	-	Set I/II
Chr. 17	S0296	Fam	55	-	Set I/II
Chr. 17	SW2441	Fam	58	1	Set IV
Chr. 17	SW840	Fam	53	-	Set I/II
Chr. 17	SW1920	Tet	58	15	Set VI
Chr. 17	SW1031	Fam	58	23	Set IV
Chr. 17	S0014	Tet	60	-	Set III
Chr. 17	SW2431	Fam	60	51	Set IV
Chr. 18	SW1808	Hex	60	14	Set VI
Chr. 18	SW1023	Fam	55	-	Set I/II
Chr. 18	SW787	Fam	55	-	Set I/II
Chr. 18	S0120	Fam	58	-	Set III
Chr. 18	SWR414	Tet	55	-	Set IV
Chr. X	SW949	Tet	58	17	Set III
Chr. X	SW980	Fam	55	27	Set I/II
Chr. X	SW2534	Hex	60	45	Set IV
Chr. X	SW2126	Tet	55	-	Set VI
Chr. X	SW2470	Hex	58	42	Set IV
Chr. X	SW2456	Hex	58	1	Set III
Chr. X	SWR1861	Hex	50	-	Set V
Chr. X	SW2476	Hex	62	-	Set III
Chr. X	SW1943	Tet	62	-	Set V
Chr. X	SW1608	Fam	58	12	Set VI
Chr. X	SW707	Hex	55	-	Set I/II
Chr. X	S0218	Tet	55	8	Set I/II
Chr. X	SW2588	Fam	62	33	Set IV

To request fluorescent primers, please contact:

Max F. Rothschild 
U.S. Pig Genome Coordinator
2255 Kildee Hall
Department of Animal Science
Iowa State University
Ames, Iowa 50011
Phone: 515-294-6202
Fax: 515-294-2401
Email: mfrothsc@iastate.edu

and indicate which primer set you are requesting. For information about each primer set, click on the Set number in the column to the right of the Table 1 (above). Please be aware of the fact that the availability of the older primer sets is limited. To know more about the flurosecent primer sets provided by the U.S. Pig Genome Coordination Program, go to the general description page.

© 2003-2024: USA · USDA · NRPSP8 · Program to Accelerate Animal Genomics Applications. Contact: Bioinformatics Team